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KMID : 0359320080480010009
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Korean Journal of Veterinary Research
2008 Volume.48 No. 1 p.9 ~ p.15
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Determination of eugenol in Eugenia caryophyllata by high-performance Liquid chromatography with photodiode array detection and method validation
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Yun Hyeong-Jun
Yun So-Mi
Lee Myung-Heon
Son Seong-Wan
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Abstract
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A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at 60oC for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients (r2> 0.9999) from 0.0125 ~ 1 μg/ml. The limit of detection per sample injection (20 μl) was 0.81 ng/μl and limit of quantification was 2.47 ng/ μl. The method showed good intra-day precision (%RSD 0.08 ~ 0.27%) and inter-day precision (%RSD 0.32 ~ 1.19%).
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KEYWORD
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clove, eugenol, HPLC, validation
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